Sterilization & Cleaning

Alcohol Lamp

An open flame for sterilising loops and needles on the spot.

Illustration of a alcohol lamp
Typical specifications
FuelDenatured alcohol or ethanol
Flame temperatureRoughly 400 – 800 °C at core
Burn time2 – 4 hours per fill
WickCotton, replaceable
UseLoops, needles, scalpels
StyleGlass body with metal collar
Sterilization & CleaningSmall batches

Overview

An alcohol lamp provides a small, steady, soot-free flame that is ideal for sterilising inoculation loops and needles. The flame also creates a rising convection current that gently pushes particles away from your work area, which is why flames are still used alongside a still-air box rather than replaced by it.

Primary stage: Inoculation

Where it fits in the grow

Used during agar transfers and syringe preparation.

Safety & handling

Never refill a lamp while it is lit or still hot, and never leave an open flame unattended. Store fuel in a clearly labelled container away from the lamp.

Practical advice

Hold the loop in the cool upper cone of the flame until it glows red, not the wick itself — the wick region deposits soot that will contaminate your plate.

Related

Tools that pair with this